Carbohydrates gold-labeled

Fiebrig, L, Varum, K.M., Elarding, S.E., Davis, S.S., and Stokke, B.T. Colloidal gold and colloidal gold labelled wheat germ agglutinin as molecular probes for identification in mucin/ chitosan complexes, Carbohydr. Polym., 33 91-99 (1997). 

Carbohydrates were cy to chemically detected in hydrogenosome membranes using the periodic acid-thiosemicarbazide-silver proteinate technique (Fig. 6a) and gold-labeled lectins, such as WGA (Fig. 6b) (Benchimol... 

Fig. 6 Carbohydrates in hydrogenosomes. Monocercomonas sp. after the Thiery technique (a). The hydrogenosomal membranes are positive for carbohydrates, b T. foetus cryosection labeled with gold-conjugated WGA. Hydrogenosome (H) shows that the membrane lining the peripheral vesicle, but not other portions of the organelle, is labeled. Bars = 100 nm. (Fig. 6a from Diniz and Benchimol 1998 Fig. 6b from Benchimol et al. 1996a)...

Lectins, or proteins with specific binding sites for carbohydrates, can be used as targeting molecules to localize particular glycoconjugates such as glycoproteins or glycolipids on cell surfaces (Fig. 373). Labeled with gold particles, lectins are important probes for detection of cell surface components and intracellular receptors and in immunological or biochemical assay procedures (Bog-Hansen et al., 1978 Kimura et al., 1979 Nicolson, 1978 Roth, 1983 Benhamou et al., 1988 Nakajima et al., 1988). 

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