Silver protein

Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. (1996). Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68, 850—858. 

This procedure is not solely specific for carbohydrate side chains of proteins. Unglycosylated proteins may also be stained. To identify glycosylated proteins, the sample should be run in at least two identical lanes cut the gel and stain a lane with the common protein silver stain (Protocols 2.4.2.1 to 2.4.2.4) and the other lane by the described method. Compare pattern and intensity to identify glycoproteins. Glycosylated macromolecules are also stainable with Schiff s reagent (Protocol 2.4.4.1), but with less sensitivity. 

Heavy metals Denature proteins Silver nitrate is used to prevent gonococcal infection, mercury compounds are used to disinfect skin and objects, copper inhibits algal growth, and selenium inhibits fungal growth... 

There are several polymers among the active substances. Classical drugs include disinfectant protein silver (Argentum proteinicum) and colloidal silver (Argentum colloidale), which are mentioned in the pharmacopoeias of several countries. Dextran (its sterile solution) is used for plasma supplementation. Polypeptide and macropeptide hormones (e.g. hormones produced in the hypophysis, pancreas, and ovaries), enzymes (e.g. pepsin, pancreatin), polymer antibiotics (e.g. polymixin, vancomycin) must be mentioned. There are several polymer molecules among cytostatic agents, too [13]. 

Fig. 3 Affinity purification of BUR-binding proteins. Silver staining analysis as performed for 1 /ig protein of a crude cell extract prepared from the breast carcinoma cell line SK-BR-3 (lane 2) and 10 ng each of column-purified pll4 from the SK-BR-3 extract (lane 3), SATBl from mouse thymus extract (lane 4), and nucleolin from human KS62 cells (lane 5). Lane 1, Molecular size marker.

Merril, C. R., and Pratt, M. (1986) A rapid sensitive protein silver stain and assay system for proteins on membranes. Anal. Biochem. 156, 96-110. 

After a lack of interest in these blends for some 20 years, there were some new developments in this field. Thus, protein silver was incorporated in NR to produce antimicrobial latex compositions." Proteins were also used to produce conductive rubber." Collagen protein fibres were incorporated in NR to produce studless tyres exhibiting good properties at low temperatures." Proteins were also cited as potential biofillers for accelerating the biodegradability of blends mainly composed of polyolefins and rubbers." ... 

Imaging/Labeling Applications Antibodies, " bacteria chromosomes histone deacetylase Hodgkin and Reed Sternberg (HRS) cells microorganisms nucleotides/nucleic acids peptides/proteins silver particles T lymphoc5de antigens " ... 

---