Carbohydrates cross-linking methods

In order to coat completely the peroxidase with albumin, the knowledge of the enzyme structure is needed to choose the best cross-linking method. Welinder (17) published the amino-acid sequence. Six residues in 308 are lysine. About 56 carbohydrate residues are grouped in 8 chains. The total molecular weight is 44,000. 

The basic mechanism of separation of carbohydrates is by ligand exchange chromatography but is quite similar to ion-exclusion chromatography described earlier in this chapter for weak organic and inorganic acids. The column contains fully sulfonated polystyrene polymer beads cross-linked with polydivinylbenzene. The polymers are fully hydrated and contain occluded water within the gel polymer matrix, just as in ion-exclusion polymer beads. Analytes partition between the occluded water within the bead matrix and the mobile phase. Water is most often used as the mobile phase and the detection method is most often refractive index. 

Shi, A. Li, D. Wang, L. Adhikari, B. Rheological properties of suspensions containing cross-linked starch nanoparticles prepared by spray and vacuum freeze drying methods. Carbohydr. Polym. 2012, 90 (4), 1732-1738. 

Chapter 42 - In the course of the work on value addition of Indian seaweeds in the laboratory, modifications of seaweed polysaccharides by various methods such as grafting of these carbohydrate polymers with different substrates, blending and cross linking were embarked upon. This has resulted in hydrogel materials exhibiting improved properties and... 

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