Capillary thalidomide

The use of antibiotic-based CSPs has been reported in capillary electrochromatography (CEC) for chiral resolution [60]. Teicoplanin CSP covalently bonded to silica gel was used to resolve the enantiomers of tryptophan and dinitrobenzoyl leucine by CEC [61]. Good levels of enantioselectivity were obtained with optimized separations. Vancomycin covalently bonded to silica gel was also evaluated in CEC for the chiral resolution of thalidomide and jS-adrenergic blocking agents under all the three mobile phase modes. The... 

Fig. 9.11. Enantiomer separation of thalidomide on a vancomycin chiral stationary phase by CEC under a slight overpressure (10 bar). Conditions 75 pm x 35.5 cm capillary mobile phase, methanol-acetonitrile-glacial acetic acid-triethylamine (80 20 2 0.2, v/v/v/v) 20 kV, UV detection, 220 nm. Reproduced from [52], with permission.

Fig. 1 Simultaneous separation and enantioseparation of thalidomide, 5-hydroxythalidomide, and 5 -hydroxythalidomide in CE using polyacrylamide-coated capillary and a mixture of 15 mg/mL sulfobutyl (4.0)-/3-CD and 10 mg/mL (3-CD as the chiral carrier.

Meyring, M. Chankvetadze, B. Blaschke, G. Enantioseparation of thalidomide and its hydroxylated metabolites using capillary electrophoresis with various cyclodextrins and their combinations as chiral buffer additives. Electrophoresis 1999, 20, 2425-2431. 

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