Fluorescently labeled calmodulin

Studies of calcium release. Calmodulin mediates many of the regulatory functions of calcium ions. Fluorescently labeled calmodulin is used to study the in vivo behavior of the protein (e.g. in the mammalian mitotic spindle) In vitro, fluorescently labeled calmodulin has been used for ... 

Allen, MW, Urbauer, RJB, Zaidi, A, Williams, TD, Urbauer, JL, and Johnson, CK, Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule espeiiments. Analytical Biochemistry 325 (2004) 273-284. 

A novel pump-damp-probe method (PDPM), which allows the characterization of solvation dynamics of a fluorescence probe not only in excited but also in the ground states has been recently developed (Changenet-Barret, 2000 and references therein). In PDPM, a pump produces a nonequilibrium population of the probe excited, which, after media relaxation, is simulated back to the ground states. The solvent relaxation of the nonequlibrium ground state is probed by monitoring with absorption technique. The inramolecular protein dynamics in a solvent-inaccessible region of calmodulin labeled with coumarin 343 peptide was examined by PDPM. In the pump-dump-probe experiments, part of a series of laser output pulses was frequency-doubled and softer beams were used as the probe. The delay of the probe with respect to the pump was fixed at 500 ps. 

O. Kogi, A. Fukushima, S. Ishizaka, N. Kitamura, Fluorescence dynamic anisotropy of spinach calmodulin labeled by a fluoreseein chromophore at Cys-26, Analytical Sciences 18, 689-691 (2002)... 

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