Pump-damp-probe method

A novel pump-damp-probe method (PDPM), which allows the characterization of solvation dynamics of a fluorescence probe not only in excited but also in the ground states has been recently developed (Changenet-Barret, 2000 and references therein). In PDPM, a pump produces a nonequilibrium population of the probe excited, which, after media relaxation, is simulated back to the ground states. The solvent relaxation of the nonequlibrium ground state is probed by monitoring with absorption technique. The inramolecular protein dynamics in a solvent-inaccessible region of calmodulin labeled with coumarin 343 peptide was examined by PDPM. In the pump-dump-probe experiments, part of a series of laser output pulses was frequency-doubled and softer beams were used as the probe. The delay of the probe with respect to the pump was fixed at 500 ps. 

Note The principal objective of shop verification by response to unbalance is to verify the existence of a critical speed (vibration peak) within tolerance of the calculated value, or if the analysis predicted a highly damped critical speed, the absence of a vibration peak within tolerance of calculated value. Shop verification by this method is feasible only for pumps that have sleeve bearings and are furnished with proximity probe pairs at each journal bearing. 

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