Calmodulin binding protein

Finally, a 150 kDa calmodulin binding protein, caldesmon is found bound to actin filaments at low calcium concentrations. The ratio of caldesmon to actin molecules seems to vary from 1 20 in the aorta to as much as 1 200 in chicken gizzard. It has been reported and disputed that caldesmon crosslinks actin filaments into large bundles. 

Volume 139. Cellular Regulators (Part A Calcium- and Calmodulin-Binding Proteins)... 

Bouche N, Yellin A, Snedden WA, Fromm H, Plant-specific calmodulin-binding proteins, Ann Rev Plant Biol 56 435M66, 2005. 

Deactivation of calmodulin-dependent enzymes may involve calmodulin-binding proteins such as calcineurin,229 although calcineurin has been shown to possess phosphatase activity.230... 

Fairley, J. A., Scott, G. A., Jensen, K. D., Goldsmith, L. A., and Diaz, L. A. (1991). Characterization of keratocalmin, a calmodulin-binding protein from human epidermis./ Clin. Invest. 88, 315-322. 

Means, A. R., Cruzalegui, F., LeMagueresse, B., Needleman, D. S., Slaughter, G. R. and Ono, T., 1991, A novel Ca2+/calmodulin-dependent protein kinase and a male germ cell-specific calmodulin-binding protein are derived from the same gene, Mol Cell Biol, 11, pp 3960-3971. 

Structures of the motor domains of three different kinesin-14 proteins, kinesins with a C-terminal motor domain, have been determined so far (1) the Drosophila nonclaret disjunctional gene product (DmNcd), (2) the yeast kinesin-like nuclear fusion protein (ScKar3), and (3) the kinesin-like calmodulin binding protein from potato (PoKCBP). 

Flanagan, S. D. and Yost, B. (1984) Calmodulin-binding proteins visualization by 125I-Calmodulin overlay on blots quenched with Tween 20 or bovine serum albumin and poly(ethylene oxide). Anal. Biochem. 140, 510-519. 

Calmodulin Sepharose Calmodulin Calmodulin-binding proteins, neurotransmitters Pharmacia... 

Sobue, K., Muramoto, K., Fujita, M. and Kakiuchi, S. (1981). Purification of a calmodulin-binding protein from chicken gizzard that interacts with F-actin. Proc. Natl. Acad. Sci. USA 78, 5652-5655. 

Noland, T.D., Van Eldik, L.J., Garbers, D.L., and Burgess, W.H. (1985). Distibution of calmodulin and calmodulin-binding proteins in membranes from bovine epididymal spermatozoa. Gamete Res. 77 297-303. 

Wasco, W.M., Kincaid, R.L., and Orr, G.A. (1989). Identification and characterization of calmodulin-binding proteins in mammalian sperm flagella. J. Biol. Chem. 264 5104-5111. 

An azido-12->I-calmodulin derivative suitable for labeling calmodulin binding proteins by photoaffinity has recently been described (45). Azido-calmodulin retained the ability to interact with and activate the calmodulin-dependent phosphodiesterase in a Ca +-dependent manner. Upon photolysis calmodulinbinding subunits of the enzyme formed 1 1 crosslinked complexes with the derivative. Crosslinked products were not obtained with incubations performed with EGTA or in large excess of unmodified calmodulin. Following photoaffinity labeling the calmodulin-dependent ATPase from red cells was found to be irreversibly activated by the derivative. 

Since CD was first isolated as a Ca +-calmodulin binding protein (Sobue et al., 1981), it has been supposed that Ca2+-dependent regulation of the thin filaments is due to Ca2+ binding to calmodulin (CM), thereby reversing inhibition (Smith et al, 1987 Marston and Smith, 1985 Sobue et al., 1982). This may readily be incorporated into the regulatory model (Fig. 

Ca + <1> (inhibition in millimolar, activation in micromolar range) [25] calcineurin <1> (i.e. calmodulin-binding protein, blocks activation by calmodulin) [29]... 

Deactivation of calmodulin-dependent enzymes may involve calmodulin-binding proteins such... 

Glenney, J. R., and Weber, K. (1980) Calmodulin-binding proteins of the microfilaments... 

Also, H,-receptor stimulation may amplify H2 effects via intracellular Ca + activation of adenylate cyclase by a calmodulin-calmodulin-binding protein complex in the catalytic subunit of the enzyme (Garbarg and Schwartz, 1988). 

FIGURE 2. Post-receptor events initiated by H2- and H,-receptor activation by histamine (HIS). Adenylate cyclase (AC) stimulation occurs directly via H2-receptor activation or possibly indirectly via a calmodulin-calmodulin binding protein complex. Membrane inositol phospholipid (IPL) hydrolysis to inositol trisphosphate (IP3) is triggered by phospholipase C (PC) activation following H,-receptor stimulation. 

Saucerman JJ, Bers DM (2012) Calmodulin binding proteins provide dmnains of local Ca2+ signaling in cardiac myocytes. J Mol Cell Cardiol 52(2) 312-316... 

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