Caliper chip

Figure 4.8 Schematic of the commercial 110 Caliper chip micro reactor for performing organic...

P 39] Protocol for single-run processing reservoirs of the 110 Caliper chip were filled with the following solutions [18] 0.10 M solutions of rac-citronellal and a synthesized aromatic aldehyde in methanol/water (80 20) and 0.12 M solutions of 1,3-dimethylbarbituric acid and Mel drum s acid in methanol/water (80 20) with 10% molar catalyst efhylenediamine diacetate (EDDA). 

OS 57] [R 6] [P 40] One of the first examples of parallel multi-reaction was performed in a Caliper chip [18], By 2 x 2 combinations of two aldehydes and two 1,3-diketones, four cycloadducts were generated simultaneously in one run on one chip (Figure 4.71). The conversions were comparable to those for the single runs, with one exception. Also, cross-contamination was observed. It ranged from a few percents to about 50%. It should be pointed out that despite these initial drawbacks the demonstration of multi-reaction feasibility is a further valuable step in micro channel processing. 

Figure 4.71 Design of a commercial Caliper chip and assignment of fluidic ports to reactant and product solutions used for carrying out (see [OS 57]) [18. ...

The etched micro channels have different widths for more stable flow, e.g. to avoid a dependence on capillary forces in the reservoirs [18]. The glass chip is glued to a polymer caddy for interfacing with a multiport control device, the Caliper 42 Workstation. 

A 10 pi volume of the aldehyde was placed in three reservoirs and 10 pi of the 1,3-diketone in three other reservoirs [18]. The chip was inserted into the interfacing device and a script for defining pressures was set in the multiport control using a Caliper 42 Workstation . A 30 min run was carried out. An eight-peristaltic pump system was used as pressure or vacuum source. N o active quenching was performed, but it was assumed that by dilution in the collection reservoir reaction was stopped or at least notably slowed. 

Caliper Technologies Corporation, Palo Alto, California, is working toward creating a miniature chemistry laboratory about the size of a toaster that can be used with plug-in chip-based laboratories. Various chips would be furnished with the unit that would be appropriate for different types of analyses. The entire unit would be connected to a computer to collect and analyze the data. There is even the possibility that these laboratories could be used in the home to perform analyses such as blood sugar and blood cholesterol and to check for the presence of bacteria such as E. coli and many others. This would revolutionize the health care industry. ... 

Plastic chips such as this one made by Caliper Technologies are being used to perform laboratory procedures traditionally done with test tubes. 

Today, Caliper Life Sciences, MA, USA [255] and Agilent Technologies, CA, USA [256] offer microfluidic chips for DNA and Protein analysis. Liquid propulsion is provided via electroosmosis and combined with capillary electrophoretic separation. The sample is electroosmotically transported and metered inside the chip, then separated via capillary electrophoresis and analysed by fluorescence detection. (Fig. 14). The whole assay is performed within minutes, instead of hours or days. 

Caliper/Agilent markets DNA chips which have become standard analytical tools for a number of life science laboratories. Commercial DNA chips are also available from companies such as Affymetrix and GE Healthcare. Microfluidic chips for carrying out purification processes for nucleic acids have been reported Cell isolation and lysis as... 

DNA chips aie provided by more than 20 companies (e.g., Affymetrix, A Gene, Amersham Pharmacia Biotech, Genometrix, Genomic Solutions, Hyseq Incyte Pharmaceuticals, Nanogen, etc.), protein chips by one company (Ciphergen) and microfluidic chips by several companies [Aclara Biosciences, Caliper (Cellomics), Cepheid and Orchid Biocomputer]. 

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