Calibration of Internal Standard to Acyl-ACP

Quantification of elongated material in reactions required the addition of alkyl-ACP internal standard, which generates an ion at m/z 318.2 following PPant ejection. In order to ascertain whether the alkyl-ACP could be used as an accurate measure of the concentration of elongated material, a calibration between alkyl- and acetyl-ACP was performed. The calibration was conducted over a range of concentrations 

The cahbration yielded a linear, 1 1 relationship over the range of 0.5-20 p.M, between theoretical and measured values of the acetyl-ACP, using alkyl-ACP as an internal standard (Fig. 6.6). This result meant that direct quantification of elongated material in the reaction was possible by addition of alkyl-ACP as an internal standard. 

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