Calibration central composite design

The ability to detect Cu2+, Cd2+ and Pb2+ with different electrodes modified in the same way but with different peptides selective for different metals opens the door to fabricating an electrode array for more than one metal ion. To explore that possibility an electrode array with four elements was employed where each electrode was modified with thioctic acid. The four elements were electrodes modified with (a) no peptide (that is thioctic acid alone), (b) Gly-Gly-His, (c) GSH and (d) human angiotensin I (see Fig. 10.5). The electrode array was calibrated by preparing 16 different cocktails of Cu2+, Cd2+ and Pb2+ according to a central composite design [53]. OSWV for each electrode for one of the calibration solutions (0.100 pM Cu2+, 3.00 pM Cd2+ and 0.600 pM Pb2+ in 50 mM ammonium acetate at pH 7.0) are shown in Fig. 10.6. The two features apparent from Fig. 10.6 are that there are only two main peaks due to the overlap of the electrochemistry from... 

Figure 12.4 The spatial representations of four different types of experimental designs that are useful for process analyzer calibration (A) full-factorial, (B) Box-Behnken, (C) face-centered cube, and (D) central composite.

Figure 6.6 Schematic procedure of a multivariate calibration. Data are taken from the NIR spectroscopic monitoring of the nitration of toluene conducted in microreactors using pure nitric acid as nitrating agent [10]. Note that model optimization is an iterative approach that requires the multiple application of steps (c) and (d). (a) Definition of an experimental design within the investigated parameter space. Here, a central composite plan is presented, (b) Experiments in accordance with the design spectrum generation in a flow-through cell and...

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