Calendulas flos

Fig. 7j8 Calendulae flos Marigold flowers Calendula officinalis L. Asteraceae...

Calendulae flos (2) is characterized by pairs of yellow-orange isorhamnetin and qucrcc-tm glycosides. The major zones are due to rutin (R, 0.4) and narcissin (R, 0.45), isorhamnetin- and quercetin-3-O-glucoside (R, 0.6-0.7) and isorhaninetin-rutinosyl-ghicoside at R, 0.2. 

Hcterothecae flos (3) has a similar TLC pattern with Arnicae flo.s (4). Together with Calendulae flos (2), it counts as an adulterant of Arnicae flos. The adulterants can be easily detected by the presence of rutin (Rf — 0.4/T1/T2) ( see also Fig. 7). 

Cacti flos (2) contains more monoglycosides (Rf 0.6-0.7), e.g. cacticin an isorhamnetin-3-0-g ucoside at R, 0.7, while Calendulae flos (1) has more triglycosides (Rf 0.2-0.25) and additional blue fluorescent zones of phenol carboxylic acids at the solvent front and chlorogenic acid at R, 0.5 (T1). 

From the drug flos Calendulae Calendula officinalis L.), two flavonoids were isolated by bioassay-guided fractionation [226]. They showed potent antimicrobial activity against the following microorganisms Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Candida monosa and Sarcinia lutea. 

Heisig, W., M. Wichtl Bestimmung pflanzhcher Glycoside. Methodenkombination von zweidimensionaler DC und HPLC. I. Inhaltsstoffe aus Calendulae officinalis flos , Dtsch. Apoth. Ztg. 130, 2058-2062 (1990)... 

Fig. 7A Calendulae (1) and Cacti flos (2) are both characterized by orange-yellow quercetin and...

B Saponins in Calendulae and Primulae flos For the separation of saponin glycosides, the... 

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