Calcitonin cell culture

The biochemical mechanisms by which cAMP-dependent phosphorylation leads to enhanced IR-PTH release remain to be determined. It is of interest, however, that isoproterenol activates phosphorylation of proteins of similar molecular weight in the rat parotid gland (22), while glucagon stimulates phosphorylation of a protein of molecular weight 19,000 in calcitonin-secreting cultured cells from a medullary carcinoma of the rat thyroid (22) It is conceivable that in all three tissues, activation of exocytosis results from a cAMP-dependent phosphorylation of a critical cellular substrate. 

Ausiello DA, Hall DH, and Dayer JM. Modulation of cyclic AMP-dependent protein kinase by vasopressin and calcitonin in cultured porcine renal LLC-PK1 cells. Biochem J 186 773-780,1980. 

The gradual decline of the concentration of pulmonary neuroendocrine cell immunoreactive calcitonin in newborn hamster lung cells cultured over 4 weeks was prevented when the medium was supplemented with nicotine for 3 weeks (Nylen et al. 1993). 

M14. Mason, R. T., Peterfreund, R. A., Sawchenko, P. E., Corrigan, A. Z., Rivier, J. E., and Vale, W. W., Release of the predicted calcitonin gene-related peptide from cultured rat trigeminal ganglion cells. Nature 308,653-655 (1984). 

DeBustros, A. Lee, R. Y. Compton, D. Tsong, T. Y. Baylin, S. B. Nelkin, B. D. Different cis-acting DNA elements regulate expression of the human calcitonin gene in cultured lines of lung carcinoma and medullary thyroid carcinomas. Mol. Cell Biol. 10, 1773-1778. 

Goldring, S.R., Sayer, J.M., Ausiello, D.A. and Krane, S.M. (1978). A cell strain cultured from porcine kidney increases cyclic AMP content upon exposure to calcitonin or vasopressin. Biochem. Biophys. Res. Commun., 83, 434-40... 

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