Caco-2 cells model

FIGURE 3.2.1 In vitro digestion/Caco-2 cell model combination approach to assess carotenoid bioavailability. LCM = large chylomicrons. SCM = small chylomicrons. VLDL = very low density lipoproteins. 

S In Vitro Digestion/Caco-2 Cell Model Combination Approach... 

Ferruzzi, M.G., Failla, M.L., and Schwartz, S.J., Assessment of degradation and intestinal cell uptake of carotenoids and chlorophyll derivatives from spinach puree using an in vitro digestion and Caco-2 cell model, J. Agric. Food Chem., 49, 2082, 2001. 

Carotenoids are highly lipophilic an active area of research concerns how carotenoids interact with and affect membrane systems (see Chapters 2 and 10). Also, the lipid solubility of these compounds has important implications for carotenoid intestinal absorption (see Chapter 17) models such as the Caco-2 cell model are being used to conduct detailed studies of carotenoid absorption/ competition for absorption (Chapter 18). The lipid solubility of these carotenoids also leads to the aggregation of carotenoids (see Chapter 3). Carotenoids aggregate both in natural and artificial systems, with implications for carotenoid excited states (see Chapter 8). This has implications for a new indication for carotenoids, namely, serving as potential materials for harnessing solar energy. 

Research Opportunities with the Caco-2 Cell Model... 

Several active transport systems that are normally found in the small intestinal enterocytes have been characterized in the Caco-2 cell model [13]. These include transport systems for glucose [32, 33], amino acids [34-37], dipeptides [38-40], vitamins [41], and bile acids [42, 43]. 

As the Caco-2 cell model contains most of the important transporters, it can be used for the study not only of passive transport mechanisms but also of mechanisms involving transporters. This is a major advantage of the Caco-2 model when... 

Wu, X., L. R. Whitfield, and B. H. Stewart. Atorvastatin transport in the Caco-2 cell model contributions of P-glycoprotein and the proton-monocarboxylic acid co-transporter. Pharm. Res. 2000, 17, 209-215. 

Petri N, Tannergren C, Rungstad D and Lennernas H (2004) Transport Characteristics of Fexofenadine in the Caco-2 Cell Model. Pharm Res 21 pp 1398-1404. 

Nguyen L, Dautrey S, Vo C, Sexton R, Morin PE, Ducharme, R. Proudlock (2001) Validation of a Caco-2 cell model for a higher throughput permeability assays. AAPS PharmSci 3(3) abstract. 

The Evolving Role of the Caco-2 Cell Model to Estimate Intestinal Absorption Potential and Elucidate Transport Mechanisms... 

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