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Burst analyses

Fluorescence Correlation Spectroscopy and Fluorescence Burst Analysis. Several nanoscopic chemical imaging approaches work very well for measurements of chemical kinetics, interactions, and mobility in solution. Fluorescence correlation spectroscopy (FCS) measures the temporal fluctuations of fluorescent markers as molecules diffuse or flow in solution through a femtoliter focal volume.54 Their average diffusive dwell times reveal their diffusion coefficients, and additional faster fluctuations can reveal chemical reactions and their kinetics if the reaction provides fluorescence modulation. Cross-correlation of the fluorescence of two distinguishable fluorophore types can very effectively reveal chemical binding kinetics and equilibria at nanomolar concentrations. [Pg.90]

Fluorescence burst analysis, a variation of FCS procedures that has an optimum configuration for simple presentation, uses a uniform nanoscopic flow channel with an optically perfect ceiling, uniform cross section, and periodic electrodes that can now be constructed by careful electron lithography techniques.55 By application of controlled electric fields, uniform plug flow of solution through... [Pg.90]

Van den Bogaart G, Krasnikov V, Poolman B (2007) Dual-colo fluorescence-burst analysis to probe protein efflux through the mechanosensitive channel. MscL Biophys J 92 1233-1240... [Pg.255]

Kapucu FE, Tanskanen JM, Mikkonen JE, Yla-Outinen L, Narkilahti S, Hyttinen JA (2012) Burst analysis tool for developing neuronal networks exhibiting highly varying action potential dynamics. Front Comput Neurosci 6 38... [Pg.370]

Kiigel W, Muschielok A, Michaelis J (2012) Bayesian-inference-based fluorescence correlation spectroscopy and single-molecule burst analysis reveal the influence of dye selection on DNA hairpin dynamics. ChemPhysChem 13(4) 1013-1022... [Pg.292]

A derivation which has found wide application in the interpretation of hydrolytic reactions and of dehydrogenases, is the so-called product burst analysis . Let us consider the condition of high substrate concentration, so... [Pg.157]

Peluso, I. Morabito, G. Riondino, S. La Farina, F. Serafini, M. Lymphocytes as internal standard in oxidative burst analysis by cytometry A new data analysis approach. J. Immunol. Methods 2012, 379, 61-65. [Pg.169]

See other pages where Burst analyses is mentioned: [Pg.552]    [Pg.91]    [Pg.168]    [Pg.139]    [Pg.10]    [Pg.10]    [Pg.12]    [Pg.12]    [Pg.201]   
See also in sourсe #XX -- [ Pg.10 , Pg.11 ]



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