Buffer action universal

Buffer action 46 Buffer capacity 48 Buffer mixture universal, (T) 831 Buffer solutions 46, (T) 831 acetic acid-sodium acetate, 49 for EDTA titrations, 329 preparation of IUPAC standards, 569 Bumping of solutions 101 Buoyancy of air in weighing 77 Burette 84, 257 piston, 87 reader, 85 weight, 86... 

Bassam Z. Shakhashiri, "Buffering Action of Alka-Seltzer," Chemical Demonstrations, A Handbook for Teachers of Chemistry, Vol. 3 (The University of Wisconsin Press, Madison, 1989) pp. 186-187. 

E. B. R. Prideaxjx and A. T. Ward have employed a mixture of several acids in order to obtain buffer solutions with a general applicability between pH s 2.0 and 12.0. The mixture used is 0.04 molar with respect to the following acids phosphoric, phenyl-acetic, and boric. The solution is neutralized with 0.2 N sodium hydroxide. The relatively small buffer action of such a universal solution decreases its general usefulness. 

The original concept of pH-buffer action arose out of biochemical studies, and the need for pH control in all aspects of biological research is now universally recognized. Unfortunately, until recently there were few suitable substances having good buffering capacity in the physiologically... 

The process of antibody optimization also requires that different temperatures of antigen retrieval be explored, and although citrate buffer at 10 mMol/litre is a good universal retrieval solution, a higher pH may be required for more capricious antigens as previously discussed (Shi et al, 1995). In addition, the synergistic action of proteolytic digestion should be routinely explored as a pre-MW and post-MW procedure. 

June 3, 1978, Lynn, Massachusetts, USA - Feb. 10, 1942 Boston, USA) Henderson studied medicine at Harvard and was Professor of Biological Chemistry at Harvard University, Cambridge, Massachusetts, from 1904 to 1942 [i]. Henderson published on the physiological role of -> buffers [ii-vii] and the relation of medicine to fundamental science. Because he and also - Hasselbalch made use of the law of mass action to calculate the - pH of solutions containing corresponding acid-base pairs, the buffer equation is frequently (esp. in the biological sciences) referred to as -> Henderson-Hasselbalch equation. 

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