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Bromide CNBr

Several highly specific chemical methods of proteolysis are available, the most widely used being cyanogen bromide (CNBr) cleavage. CNBr acts upon methio-... [Pg.135]

Cyanogen bromide (CNBr) method, for covalent ligand immobilization, 6 396t Cyanogen chloride, 3 172 Cyanoguanidine, 3 164 Cyanohydrins, 27 229... [Pg.240]

Cyanogen bromide (CNBr). (WARNING CNBr is toxic and should be handled only in a fume hood.)... [Pg.96]

Cyanogen bromide (CNBr)-activated Sepharose 4B (see Chapter 10, Section 3 4 1.)... [Pg.114]

Figure 1 Strategy for cloning a peptide-coding sequence (CDS) as tandem repeats in the vector pET31b. The resulting fusion protein, comprising the ketosteroid isomerase (KSI), peptide repeats, and His-tag, is targeted to inclusion bodies. The fusion protein can be recovered and cleaved, in this case, with cyanogen bromide (CNBr) which acts at the methionine (M) residues allowing further separation of pure peptide from the other fusion components. The cleavage by CNBr results in a C-terminal homoserine lactone (hsl) on each peptide monomer. Figure 1 Strategy for cloning a peptide-coding sequence (CDS) as tandem repeats in the vector pET31b. The resulting fusion protein, comprising the ketosteroid isomerase (KSI), peptide repeats, and His-tag, is targeted to inclusion bodies. The fusion protein can be recovered and cleaved, in this case, with cyanogen bromide (CNBr) which acts at the methionine (M) residues allowing further separation of pure peptide from the other fusion components. The cleavage by CNBr results in a C-terminal homoserine lactone (hsl) on each peptide monomer.
A nonenzymatic. chemical method for specific cleavage of polypeptides involves the reaction of cyanogen bromide, CNBr, with methionine residues ... [Pg.80]

A peptide was broken into two smaller peptides by cyanogen bromide (CNBr). and into two different peptides by trypsin (Tryp). Their sequences were as follows CNBr 1. Gly-Thr-Lys-Ala-Glu CNBr 2, Ser-Met Tryp 1, Ser-Met-Gly-Thr-Lys Tryp 2, Ala-Glu. Determine the sequence of the parent peptide. [Pg.81]

Fig. 4 Nucleic acid immobilization. Immobilization of 5 -amino-C6 modified probes onto (a) carboxylate modified latex beads (1 im) via carbodiimide reaction and (b) Cyanogen bromide (CNBr) activated Sepharose beads (100 un). (0 cyclic structure, e.g. dicyclo-hexylcarbodiimide)... Fig. 4 Nucleic acid immobilization. Immobilization of 5 -amino-C6 modified probes onto (a) carboxylate modified latex beads (1 im) via carbodiimide reaction and (b) Cyanogen bromide (CNBr) activated Sepharose beads (100 un). (0 cyclic structure, e.g. dicyclo-hexylcarbodiimide)...
A chromatographic matrix with free OH groups, such as agarose, is first activated with cyanogen bromide (CNBr). Ligand is then coupled to the... [Pg.96]

Specific cleavage can be achieved by chemical or enzymatic methods. For example, cyanogen bromide (CNBr) splits polypeptide chains only on the carboxyl side of methionine residues (Figure 4.23). [Pg.156]

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CNBr CYANOGEN BROMIDE

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