CNBr CYANOGEN BROMIDE

PTH = 3-phenyl-2-thiohydantoin DMAA = dimethylallylamine CNBr = cyanogen bromide... 

Fig. 1 Summary of the data used to establish the complete amino acid sequence of Er-1 mating pheromone. The peptides have been designated and numbered according to the type of digest ana the theoretical order in which they appear in the sequence. Designations are CNBr, cyanogen bromide T, trypsin V8, . aureus V8 protease CT, chymotrypsin. Peptiaes indicated by two numbers connected with a hyphen result from partial cleavage. Residues directly identified by automated Edman degradation and carboxypeptidase Y digestion (CP-Y) are marked by right and left arrows, respectively, residues identified by amino acid composition are indicated by dashed lines. Taken from ref. 13 and reproduced by permission of the American Society of ...

CNBr CYANOGEN BROMIDE 31.562 7.7072E-02 -1.0251E-04 7.0456E-08 -1.8400E-11 298 1500 gas... 

Analyses of the structure and modifications of the collagen molecule/chain require solubilization and fragmentation of the protein to smaller peptides. In principle, two methods can be used—non-enzymatic (chemical) or enzymatic treatment. The chemical method is cleavage by CNBr. Cyanogen bromide splits the protein molecule at specific locations—at the methionines (in this case toward the C-terminal end). In the collagen molecule, methionine is a relatively rare amino acid (some 10-20 amino acids per collagen molecule). The small number of methionine residues leads to a rather limited number of cleavage products (CNBr peptides). The profile of CNBr peptides is typical. 

CNBr, cyanogen bromide Carboxyl side of methionine... 

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