Brine shrimp toxicity

Bastos ML, Lima MR, Conserva LM, Andrade VS, Rocha EM, Lemos RR (2009) Studies on the antimicrobial activity and brine shrimp toxicity of Zeyheria tuberculosa (Veil.) Bur. (Bignoniaceae) extracts and their main constituents. Ann Clin Microbiol Antimicrob 8 16. 

Aspergillus parasiticus is an endophyte isolated from the inner bark of the redwood tree. Sequoia sempervirens (Taxodiaceae). Previously, several sequoaitones active in the brine shrimp toxicity assay were isolated from this fungal strain and more recently four new sequoaitones C — F (49—52) were isolated and found to be toxic to brine shrimp with LD50 of260 p,M, 1300 xM, 640 xM, and 260 p.M, respectively. ... 

Gerwick, W. H., Proteau, P. J., Nagle, D. G., Hamel, E., Blokhin, A., and Slate, D. L., Structure of curacin A, a novel antimitotic, antiproliferative, and brine shrimp toxic natural product from the marine cyanobacterium Lyngbya majuscula, J. Org. Chem., 59, 1243, 1994. 

Brine shrimp toxicity guided fractionation of the extracts from two mixed Fijian collections of the cyanobacteria Lyngbya majuscula and Schizothrix sp. led to the isolation of eleven novel chlorinated lipids, taveuniamides A-K 369-379. All of these metabolites showed an intriguing constellation of unsaturation (olefinic and acetylenic bonds) and chlorination at the two termini of a 15-carbon chain. The central carbon atom of the chain (C-8) was substituted in each case with an N-acetate function. Taveuniamides F 374, G 375 and K 379 were the most potent brine shrimp toxins with LD50S between 1.7 - 1.9 mg mL , while taveuniamides A-E 373-377 showed moderate activity LD50 3-5 mg mL. Taveuniamides G 375, 1 377, and J 378 were essentially inactive at a concentration of 10 mg mL. ... 

AF - antifungal AC - aniicancer AB -antibacterial, AV - antiviral AM - aniimalarial BST - brine shrimp toxicity. 

The organic mycelial extract from Media A was first partitioned between methylene chloride and water. The methylene chloride extract was resolved by normal phase CCCC (hexane/ethyl acetate/methanol/water, 1 2 2 1) into ten fi-actions. Fraction 4 was active against Sclerotinia sp., was slightly brine shrimp toxic, but was very... 

HIRE was also grown in mycological broth (without Mg3(P04)2 12H20) for 21 days unshaken (12 L), but the harvest protocol of the mycelial mat was altered. Following filtration, the mycelium was thoroughly pulverized with the Omnimixer, then soaked in methanol for 24 hr. The methanol was removed by filtration, and the mycelial marc was then extracted with chloroform/methanol (1 1). The methanol extract retained both the NMR activity and the brine shrimp toxicity. The methanol extract of the mycelial mat (10.53 g) was applied to a LH-20 size exclusion column (methanol). Seventeen fractions eluted, but all pertinent activity was concentrated in fraction 8, which was ultimately resolved by normal phase CCCC (chloroform/methanol/water, 25 24 20). Fraction 3 was brine shrimp toxic, and was found to be identical to roquefortine C... 

Ten different fungi were grown in each of the four test media both as still cultures (21 days) and shaken (6 days). The cultures were extracted as described above at time of harvest, and each extract was tested for antibacterial activity, antifungal activity, and brine shrimp toxicity. The Media D cultures were consistent with earlier growth profiles, as should be expected. 

Chiysophanic acid (= Chiywphanol) (39) Brine shrimp toxicity 112... 

Brine shrimps, toxicity of polycyclic aromatic hydrocarbons, 197,198t Bulk, mixed-layer models, description, 259... 

Isol. from the marine cyanobacterium Lyngbya majuscula. Antimitotic agent. Also possesses exceptional brine shrimp toxic and antiproliferative activities. [a]u + 86 (c, 0.64 in CHCI3). Unstable when stored neat. Stereoisomer [157319-51-2]. Curacin B C23H35NOS M 373.602... 

Isol. from the marine cyanobacterium L. majuscula. Antimitotic agent. Possesses brine shrimp toxic and antiproliferative activities. Poss. geom. isomer of Curacin A. 

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