Brain slices microscope

Brain slices were transferred and anchored to a recording chamber with a nylon grid. A peristaltic pump continually perfused the slices with ACSF (bubbled with carbogen) at a rate of 1-2 mL/min. The recording temperature was controlled at either 26 or 35 °C with an inline heating system. Slices were visualized with an upright microscope equipped with DIG optics and were displayed on a monitor using a CCD camera. 

An autopsy can also obtain tissue slices for examination with a light microscope or an electron microscope. Furthermore, on gross examination of the brain, instead of estimating tissue loss from MRI pictures, an autopsy can actually weigh and measure the brain and examine cell density under the microscope. As a result, many diseases of the brain, such as Alzheimer s, require an autopsy rather than an MRI or CT scan to make the definitive diagnosis (Caine et al., 1995). 

Garthwaite G, Garthwaite J (1988) Electron microscopic autoradiography of D-pH]aspartate uptake sites in mouse cerebellar slices shows poor labelling of mossy fibre terminals. Brain Res., 440, 162 166. 

Hokfelt T and Ljungdahl A (1971) Light and electron microscopic autoradiography on spinal cord slices after incubation with labeled glycine. Brain Res 32, 189-194... 

Iversen L. L and Bloom F E. (1972) Studies of the uptake of H-GABA and H-glycine in slices and homogenates of rat brain and spinal cord by electron microscopic autoradiography Brain Res 41, 131-143... 

Carbon fiber microcylinder electrodes (7 pm in diameter and 400 pm in length) were implanted vertically into the brains of anesthetized rats and were used to monitor electrically evoked dopamine release, as described in detail in the next section. After the electrodes were removed from the animal, the brain tissue was collected and thinly sliced in the plane perpendicular to the axis of the electrodes. When we examined the tissue sections through which the carbon fiber passed, we could find no evidence of the electrode track with the light microscope. This shows that the tissue damage caused by the fiber itself is minimal. On the other hand, tracks could be found in tissue sections through which the glass barrel of the electrode had passed because the barrels were tapered and their... 

---