Brain region receptor localization

Two CCK receptor subtypes, CCK and CCKg are known. A related receptor, the gastrin receptor, has also been described. CCK receptors predominate in the gastrointestinal tract and pancreas and are also localized in discrete brain regions. CCKg receptors predominate in the brain. A 71623... 

Hammer, R. P., Jr., Herkenham, M., Pert, C. B., and Quirion, R. (1982) Correlation of regional brain metabolism with receptor localization during ketamine anesthesia Combined autoradiographic 2-(3H)deoxy-D-glucose receptor binding technique. Proc. Natl. Acad. Sci. USA, 79 3067-3070. 

The mRNA coding for 5-HT6 receptor has been localized in the rat brain by Northern blot, PCR, and in situ hybridization (206,207,213,214) (see also Fig. 10) and the protein by immunohistochemistry (213). The first and more detailed description on the localization of mRNA coding for 5-HT6 receptor was published by Ward and co-workers (215), reporting that the main rat brain regions where this receptor is expressed is the pyramidal layer of the olfactory tubercle, islands of Calleja, nucleus accumbens, striatum, hippocampus, and piriform cortex. At moderate levels, it is expressed in other cortical areas, the olfactory bulb, some nuclei of the hypothalamus and amygdale, the habenula, and the cerebellum. No mRNA expression is found in the raphe nucleus. These results were confirmed later (204). 

The reaction is localized in brain regions which are associated with the presence of opioid receptors and with opioid sites of action. 

Acosta-Martinez M, Fiber JM, Brown RD, Etgen AM. Localization of a1B-adre-nergic receptor in female rat brain regions involved in stress and neuroendocrine function. Neurochem Int 1999 35 383-391. 

---