Bradykinin fibrinopeptides

Inserted emitter, silver glue coated, PMMA chip ESI-ITMS BSA, fibrinopeptide A, osteocalcin fragment 7-19, bradykinin (all 10 pmol/jxL) Trypsin, off-chip Nil 814... 

The human plasma metallo-protease carboxypeptidase N (CPN, arginine carboxypeptidase, anaphylatoxin inactivator, kininase I, EC 3.4.17.3) catalyzes the release of the basic amino acids lysine and arginine from the C-termini of peptides and proteins such as bradykinin and kallidin [95], the anaphylatoxins C3a, C4a, and C5a [96,97], fibrinopeptides 6A and 6D [98], hexapeptide enkephalins [99], protamine [100], and the creatine kinase MM-isoenzyme [101,102]. Its most likely physiological function is to protect the organism from the actions of potent peptides, which may escape from tissues or be released in the circulation. 

FIGURE 3.5 The calibration curve to determine mobilities of doubly protonated peptides bombesin, angiotensin II, and substance P, as well as quadruply protonated melittin. In the diagram full circles indicate the values obtained from internal calibrant peptides. These were angiotensin I + 3H+, bradykinin + 2H+, fibrinopeptide A + 2H, fibrinopeptide A + 3H neurotensin + 3H+, and GRGDS + H+. 

Bradykinin potentiating effects have been demonstrated with various compounds, some of them due to a sensitization during bioaasay with proteolytic enzymes (1), specific fibrinopeptides... 

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