Bovine haemoglobin

Bovine haemoglobin, the protein from erythrocytes which occurs as a minor component in meat and meat products, is also a precursor of opioid peptides (haemorphins). These opioid peptides are released by pepsin digestion in vitro and may also be produced by macrophages. Moreover, haemorphins have been found to decrease the tension of the guinea pig ileum in vitro (Nyberg et al., 1997). 

Standi, T. Burmeister, M-A. Horn, E-P. Wilhelm, S. Knoefel, W.T. Schulte am Esch, J. Bovine haemoglobin-based oxygen carrier for patients undergoing haemodilu-tion before liver resection. Brit. J. Anaesth 1998, 80, 189-194. 

Binding of bovine haemoglobin with non-polar -logC = 0.671ogP-t-1.96... 

In this method the keyhole limpet haemoglobin conjugate was prepared as follows Keyhole limpet haemocyanin (KLH, Calbiochem, La Jolia, CA) and bovine serum albumin (BSA, BDH Chemicals) were coupled to the adduct (2), derived from 6-bromohexanoic acid and monoquat (3), via a carbodiimide reaction, as reported previously by Niewola et al. [184], The resulting conjugates contained 662mol of Paraquat per mole of KLH and 15mol of Paraquat per mole of 6-bromohexanoic acid. The amount of Paraquat bound to the protein was determined by spectrophotometric dithionite assay for Paraquat and the protein concentration was established by a standard Lowry test. 

Shao et al. used polyethyleneimine-coated crystals to measure haemoglobin (bovine) directly in solution from 0.001 to 0.1 mgmL [142]. No interference was observed from BSA and the effect of different liquid depths over the crystal was also investigated. 

Fig. 1. Aqueous isolation of bovine erythrocyte Cu2Zn2Superoxide dismutase. Gelfiltration of the DEAE-eluate on Sephadex G-75. The fractions are assayed for Cu, Fe, snperoxide dismutase activity (NBT-assay) and electronic absorption (A s ). The observed peaks are assigned to (from left to right) catalase, haemoglobin, CujZujSuperoxide dismutase and cytochromes b/c...

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