Bone isoform alkaline phosphatase

Alkaline phosphatase is an enzyme represented by various isoforms in many tissues such as liver, bone, intestine, placenta, some tumors and in leukocytes. Addition of 1 mM levamisole to the chromogen/substrate will inhibit endogenous alkaline phosphatase activity, with the exception of the intestinal isoform. If necessary, this can be blocked with a weak acid wash, such as 0.03 0.5 N HC1 or 1 M citric acid. [Pg.43]

Alkaline phosphatase is an enzyme of the cellular membranes. Its isoforms can be found in liver, digestive tract, placenta, and some tumor tissues. Bone isoform, BALP, is a membrane enzyme of the osteoblasts. Bone, liver, and intestinal isoforms are the posttranslational modifications of the same isoenzyme exprimed by the same gene, and the difference among them lies in various ways of reaction with a saccharide component, sialic acid (M9). [Pg.277]

P9. Price, C. P., Milligan, T. P., andDarte, C., Direct comparison of performance characteristics of two immunoassays for bone isoform of alkaline phosphatase in serum. Clin. Chem. 43, 2052—2057... [Pg.292]

Brun-Heath I, Ermonval M, Chabrol E et al (2011) Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. CeU Tissue Res 343 521-536... [Pg.46]

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