Bombardment-mediated transformation

Finer, J.J., Finer, K.R., and Ponappa, T. (1999). Particle bombardment mediated transformation. Curr. Top. Microbiol. Immunol. 240 59-80. 

The most effective means of transforming plant cells is through the use of "gene guns," or bombardment-mediated transformation. DNA is coated onto 1- p, m-diameter tungsten pellets, and these microprojectiles are fired at the target... 

Particle bombardment-mediated transformation should be of consistent efficiency. 

A number of different methods for stable or transient genetic transformation of plants or plant cells have been developed [13-15]. These comprise particle bombardment, Agro actermm-mediated transformation, floral dip transformation, agrodrench, viral vectors, protoplast transformation and ultrasound. These are the main techniques for the genetic transformation of plants, and many of them have also been applied for the transformation of secondary metabolite pathways in an attempt to alter the metabolic pathways of target... 

Vongpaseuth K, Nims E, St. Amand M, Walker EL, Roberts SC (2007) Development of a particle bombardment-mediated transient transformation system for Taxus spp. cells in culture. Biotechnol Program 23 1180-1185. doi 10.1021/bp0700307... 

There are only two successfiil and repeatable methods Agrobacterium-mediated transformation of cotyledonary node explants from young seedlings or imbibed seeds and particle-bombardment transformation of somatic embryos... 

Agrobacterium-mediated stable transformation -Plant Agrobacterium-mediated stable transformation -Cell culture Transient protoplast transformation Particle bombardment-based transient expression Agrobacterium- mediated transient expression... 

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