Bisected oligosaccharides

Warner, T. G., deKremer, R. D., Sjoberg, E. R., and Mock, A. K., Characterization and analysis of branched-chain N-acetylglucosaminyl oligosaccharides accumulating in Sandhoff disease tissue. Evidence that biantennary bisected oligosaccharide side chains of glycoproteins are abundant substrates for lysosomes, J Biol Chem 260 (1985) 6194-6199. 

C5-1 antibody was produced in alfalfa, the glycan component consisted predominantly of a mature oligosaccharide comprising a core a(l,3)-fucose residue, a bisecting P(l,2)-xylose residue and two terminal GlcNAc residues (Fig. 15.4) [6],... 

Shinkawa T, Nakamura K, Yamane N, shoji-Hosaka E, Kanda Y, Sakurada M, Uchida K, Anazawa H, Satoh M, Yamasaki M, Hanai N, Shitara K. The absence of fucose but not the presence of galactose or Bisecting A-Acetylglucosamine of human IgGl complex-type oligosaccharides shows the critical role of enhancing the antibody-dependent cellular cytotoxicity. J Biol Chem 2003 278 3466-3473. 

Fig. 19. Stereo plots of space filling models of the conformation of minimum energy calculated by the GESA program for the biantennary bisected pentasaccharide, a-D-Man-( 1 -6)-[p-D-GlcAAc-(l 4)]-[ot-D-Man-(l-3)]-P-D-Man-(l-4)-p-D-Glc/VAc, of the A-type oligosaccharides. The reducing end is to the right...

Bisected biantennary core structure of the A-linked oligosaccharides... 

RP-HPLC The effect of linkage position is much more marked on RP-separations than on normal phase. Oligosaccharides containing bisecting Gn can be identified. Derivatization (e.g. 2-AP for fluorescence detection) to increase hydrophobicity required [266]... 

Bisected penta-antennary oligosaccharide found in hen ovomucoid. The GlcNAc-transferases (named with Roman numerals) are responsible for the addition of the GlcNAc residues that initiate antennae. The branches present in various multiantennary and bisected N-glycans are indicated. [Reproduced with permission from I. Brockhausen, Clinical aspects of glycoprotein biosynthesis. Crit. Rev. Clin. Lab. Sci. 30 68 (1993).]... 

Fig. 1.12 Glycosylation pattern of an human IgG-antibody. (A-C) The Asn297 N-linked oligosaccharide is a biantennary core complex (GlcNac2-Man3GINac) with variations such as a third bisecting GINac, different numbers of terminal ga-...

---