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Biotin-Diazonium Modification of DNA

Prepare diazotized p-aminobenzoyl biocytin by using the protocol outlined in Chapter 11, Section 5, but instead of starting with 2mg the biotinylation reagent dissolved in 40 il of IN HC1, use 9mg in 180pi of IN HC1. Proportionally scale up the other reactant quantities used in the protocol. After the reaction is complete, immediately adjust the pH of the final solution to 9. [Pg.990]

Purify the biotinylated DNA probe by ethanol precipitation, gel filtration, w-butanol extraction, or dialysis as discussed in previous sections. [Pg.990]

See other pages where Biotin-Diazonium Modification of DNA is mentioned: [Pg.989]    [Pg.678]    [Pg.658]   


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