P-Aminobenzoyl biocytin

Active Hydrogen-Reactive p-Aminobenzoyl Biocytin, Diazotized... 

The procedure for creating the diazonium derivative of p-aminobenzoyl biocytin and coupling to a protein or a nucleic acid is as follows. 

Mix 40 pi of the p-aminobenzoyl biocytin solution with 40 pi of the sodium nitrite solution. 

Dilute the diazonium derivative of p-aminobenzoyl biocytin with 0.2 M sodium borate, pH 8.4, to a concentration of 10 pg/ml. 

Prepare diazotized p-aminobenzoyl biocytin by using the protocol outlined in Chapter 11, Section 5, but instead of starting with 2mg the biotinylation reagent dissolved in 40 il of IN HC1, use 9mg in 180pi of IN HC1. Proportionally scale up the other reactant quantities used in the protocol. After the reaction is complete, immediately adjust the pH of the final solution to 9. 

Biotinylation of Proteins on Blots Using the Diazonium Derivative of p-Aminobenzoyl Biocytin... 

Diazonium groups are able to couple at the C-8 position of adenosine or guanosine residues, forming diazo bonds. p-Aminobenzoyl biocytin can be used in this reaction to add a biotin handle to purine bases within oligonucleotides (Chapter 8, Section 3.5). This biotinylation reagent contains a 4-aminobenzoic acid amide derivative off the... 

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