Biotin derivatives, luminescent

Chemiluminescent Immunoassay. Chemiluminescence is the emission of visible light resulting from a chemical reaction. The majority of such reactions are oxidations, using oxygen or peroxides, and among the first chemicals studied for chemiluminescence were luminol (5-amino-2,3-dihydro-l,4-phthalazinedione [521-31-3]) and its derivatives (see Luminescent materials, chemiluminescence). Luminol or isoluminol can be directly linked to antibodies and used in a system with peroxidase to detect specific antigens. One of the first appHcations of this approach was for the detection of biotin (31). 

The first class of luminescent biotinylation reagents, derived from rhenium(I) polypyridine complexes [Re(NAN)(CO)3(py-biotin-NCS)]+ (24), have been reported by Lo and co-workers [62], To investigate the amine-specific reactivity... 

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