Bioseparation systems extraction

The same advantages are exhibited by LC in comparison with techniques such as fractional crystallisation, liquid extraction, ultrafiltration and adsorption. It has already been pointed out (Section 19.6) that LC now plays a major part in bioseparations, where the technique needs to be integrated into the process train as part of a systems approach. 

Mattiasson B, Ling TGI (1987) Extraction in aqueous two-phase systems for biotechnology. In Verrall MS, Hudson MJ (eds) Bioseparation for biotechnology. Ellis Horwood, Chichester, p 270... 

The bioseparation technique which is probably the most readily adapted to modern process control techniques is extraction. Liquid-liquid extraction is a mature unit process with application in industrial-scale protein separation.30 Control techniques used on similar systems in other industrial applications should be readily adaptable to bioprocessing, the primary difficulty being the lack of data on the partitioning and related behavior of the product. 

An overview chapter by Hamel and Hunter presents the state of the art of research on bioseparations. Extraction processes using biphasic aqueous systems, liquid membranes, reversed-micellar systems, and membrane processes are all being actively studied. Significant advances in these topics, including predictive mathematical models, are presented in the first section. The second section includes several papers on affinity and other interaction techniques that are finding uses in protein purification. In the last section, we offer several reports that delineate advances in isolation and purification processes such as electrophoresis and chromatography. 

Szlag DC, Giuhano A, and Snyder SM, A low-cost aqueous two phase system for affinity extraction. In Hamel JFP, Hunter JB, and Sikdar SK, eds. Doivnstream Processing and Bioseparation, Recovery and Purification of Biological Products. ACS Symp. Ser. 419, ACS Press, Washington, 1990 pp. 71-86. 

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