Biosensor resonant mirror

Cush R., Cronin J.M., Stewart W.J., Maule C.H., Molloy J., Goddard N. J., The resonant mirror a novel optical biosensor for direct sensing of biomolecular interactions, Part I Principle of operation and associated instrumentation, Biosensors and Bioelectronics 1993 8 347-353. 

Buckle, P. E. Davies, R. J. Kinning, T. Yeung, D. Edwards, P. R. Pollard Knight, D. Lowe, C. R., The resonant mirror A novel optical biosensor for direct sensing of biomelcular interactions. Part II Applications, Biosens. Bioelectron. 1993, 8, 355 363... 

R. Cush, J. M. Cronin, W. J. Stewart, C. H. Maule, J. Molloy, and N. J. Goddard, "The Resonant Mirror A Novel Optical Biosensor for Direct Sensing of Biomolecular Interactions Part I Principle of Operation and Associated Instrumentation," Biosensors Bioelectronics 8, 347-354 (1993). 

Lathrop, A. A., Jaradat, Z. W., Haley, T., and Bhunia, A. K. (2003). Characterization and application of a Listeria monocytogenes reactive monoclonal antibody C11E9 in a resonant mirror biosensor. J. Immunol. Methods 281,119-128. 

By using a resonant mirror biosensor, the binding between YTX and PDEs from bovine brain was studied. The enzymes were immobilized over an aminosilae surface and the association curves after the addition of several YTX concentrations were checked. These curves follow a typical association profile that fit a pseudo-first-order kinetic equation. From these results the kinetic equilibrium dissociation constant (K ) for the PDE-YTX association was calculated. This value is 3.74 p,M YTX (Pazos et al. 2004). is dependent on YTX structure since it increases when 44 or 45 carbons (at C9 chain) group. A higher value, 7 p,M OH-YTX or 23 p,M carboxy-YTX, indicates a lower affinity of YTXs analogues by PDEs. 

Pazos, M.I, Alfonso, A., Vieytes, M., Yasumoto, T, and Botana, L. 2004. Resonant mirror biosensor detection method based on yessotoxin-phosphodiesterase interactions. Analytical Biochemestry 335, 112—118. 

Skladal, P. and Horacek, J. (1999) Kinetic studies of affinity interactions comparison of piezoelectric and resonant mirror-based biosensors. Anal. Lett. 32, 1519-1529... 

Pazos, M.J. et al., Study of the interaction between different phosphodiesterases and yessotoxin using a resonant mirror biosensor, Chem Res Toxicol 19, 794, 2006. 

Pazos, M. et al.. Kinetic analysis of the interaction between yessotoxin and analogs and immobilized phosphodiesterases using a resonant mirror optical biosensor, Chem Res Toxicol, 18, 1155, 2005. 

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