Bioprocesses chromatographic separation

The offline analysis of selected metabolites of the cells during a bioprocess can be performed by MS, NMR spectroscopy, or chromatographic separation procedures. The NMR technique is based on the specific response of atomic nuclei that are exposed to a magnetic field. This technique is able to resolve the structures of small biomolecules and thus to determine selected metabolites during a bioprocess or for metabolic flux analyses. Advanced MS techniques allow a high throughput analysis of complex mixtures of metabolites by the determination of the specific masses of the metabolites. 

---