Biomolecular assay formats

Trubetskoy, O.V., Gibson, J.R. and Marks, B.D. (2005) Highly miniaturized formats for in vitro drug metabolism assays using vivid fluorescent substrates and recombinant human cytochrome P450 enzymes. Journal of Biomolecular Screening, 10 (1), 56-66. 

SPR affinity biosensors have been developed to detect an analyte in a variety of formats. The choice of detection format for a particular application depends on the size of target analyte molecules, binding characteristics of available biomolecular recognition element, and range of concentrations of analyte to be measured. The main detection formats used in SPR biosensors include direct detection (Fig. 11), sandwich assay (Fig. 12) and inhibition assay (Fig. 13). 

Kolb, A., Neumann, K. (1997). Beyond the 96-well Microplate Instruments and Assay Methods for the 384-well Format. Journal of Biomolecular Screening, in press. 

Figure 5.11 Photon Count histogram of 5 nM unbound conjugate (estradiol-Tamra) (a) in the presence of 1 (xM competitor (estradiol) or (b) in the absence of competitor at full complex formation with nanoparticles. Total number of photons counted within the measurement time was 1,848,127 (a) and 1,504,390 (b). Reprinted with permission from SchaertI ef a/., A novel and robust homogeneous florescence-based assay using nano particles from pharmaceutical screening and diagnostics. Journal of Biomolecular Screening 5 (2000) 227-237. Copyright 2000 Sage Publications, Inc.

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