In Vivo Bioimaging

By introducing propeller-like starburst triphenylamine as a strong donor to increase the emission wavelength and hinder the ACQ effect (Figure 5.9), two DPP-based red/NIR compounds (DPP-1 and DPP-2) exhibit both large two-photo absorption (2PA) cross-sections and excellent AIE properties. The large Stokes shifts (AA > 3571 cm ) are also helpful for bioimaging in vivo. Compared with DPP-1, incorporation of the extended n-deficient... 

Liu, Q Feng, W and Li, F. (2014) Water-soluble lanthanide upconversion nanophosphores synthesis and bioimaging in vivo. Coord. Chem. Rev., 273-274, 100-110. 

The strategy described has been demonstrated by the in vivo labeling of proteins in both bacterial and mammalian systems thereby making it potentially useful for future bioimaging and proteomics applications [163],... 

Kamimura M, Kanayama N, Tokuzen K, Soga K, Nagasaki Y (2011) Near-infrared (1550 nm) In vivo bioimaging based on rare-earth doped ceramic nanophosphors modified with PEG-b-poly (4-vinylbenzyl phosphonate). Nanoscale. doi 10.1039/ClNR10466G... 

CARS microscopy has emerged as a highly sensitive analytical tool for vibrational bioimaging, predominantly, of lipids in membrane model systems [69, 81-84], live unstained cells [85-95, 43], and both ex vivo and in vivo tissues [26, 96-103, 43]. Examples of CARS imaging applications in the physical and material sciences include the study of fracture dynamics in drying silica nanoparticle suspensions [104], patterned polymeric photoresist film [105], drug molecules in a polymer matrix [106], and liquid crystals [107, 108],... 

Bifunctional biolabels with magnetic and luminescent properties are highly desirable for in vitro and in vivo bioimaging. There are several strategies to use NPs to comprise such biolabels, such as core/shell NPs, for example, the magnetic cores of iron oxide doped with cobalt and neodymium and luminescent shells of Gd203 Eu (Dosev et al., 2005,2007). 

Nyk, M., Kumar, R., Ohulchanskyy, T.Y., etal. (2008) High contrast in vitro and in vivo photoluminescence bioimaging using near infrared to near infrared up-conversion in Tm " and Yb + doped fluoride nanophosphors. Nano Letters, 8, 3834—3838. 

Covalently chitosan-capped InGaP-ZnS QDs of 30-50 nm size were first used for in vivo bioimaging by Tabrizian s group. The chitosan coating not only enhanced biocompatibility, but also improved cellular uptake, and allowed deeper tissue penetration with respect to PEGylated QDs. 

Prepared doped NIR QDs with tunable wavelength and lifetime were wrapped into microbeads. Based on fluorescence lifetime and wavelength characteristics, a novel QD-based barcode was developed.The fluorescence lifetime imaging mode (FLIM) based on QDs with small size (3.5 nm), long lifetime (1 ps), NIR emission (720 nm), and pH sensitivity was also used for in vivo bioimaging and biotracking. ... 

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