Biofocus approach

In the BioFocus approach, the analysis was driven by the observed SAR of the systems and in the above case by some elegant experimental biology. More formally Thematic Analysis is a superset of the above approach as it describes the recognition processes in all-hehcal environments and hence is able to be extended to Family B and C GPCRs and also ion channels. 

Noteworthy in this perspective is the recent work done at Pfizer and Biofocus (see Section 5.5), where, based on analysis of sequence data, mutation data, and physicochemical properties of the ligands, approaches were outlined for discovering sequence patterns characteristic of specific ligand classes. Pfizer applied the approach successfully to the construction of a sequence motif characteristic of monoamine GPCRs [74],... 

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