Mosquitocidal bioassay

Insecticidal assay. The bioassay for insecticidal properties was conducted on 4th instar mosquito larvae, Aedes aegyptiy reared from the mosquito eggs (University of Davis California Straw, courtesy of Dr. David Grant) and on brine shrimp. Anemia salina Leach (obtained loc y). For the mosquitocidal assay, 10 larvae were placed in 975 fil distilled water and 25 pi of test compounds in DMSO were added and mixed at room temperature. The number of dead larvae was recorded at 2, 4 and 24 h intervals. The control tube containing 10 larvae received 25 pi of DMSO alone and mortality was recorded as in the case of test compounds. 

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