Gypsy moth bioassay

Thyagaraja B. S. and Raina, A. K. (1994) Regulation of pheromone production in the gypsy moth, Lymantria dispar, and development of an in vitro bioassay. J. Insect Physiol. 40, 969-974. 

Neuroendocrine control of invertebrate development was first demonstrated by Kopec CL2) with neck ligation and brain extirpation-reimplantation studies using the gypsy moth, Lymantria dispar (Lepidoptera Lymantriidae). His demonstration of a "pupation factor", origmating in the bi in, provided the impetus for identification of numerous invertebrate physiological processes controlled by peptide neurohormones (3.41 In insects, identified processes now number over thirty (5). Knowledge of these processes has provided for the development of bioassays used in the isolation of neuropeptides. Since 1975, over fo insect peptide neurohormones have been isolated and sequenced (5-81... 

Fractions were bioassayed in field traps 18) or in the laboratory 4), In the latter method, gypsy moth males imprisoned by their wings and exposed to the scent on a rod or filter paper exhibited curving of the abdomen and copulatory attempts. 

Gypsy Moth and Forest Tent Caterpillar Bioassays... 

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