Bioaffinity system

Keywords Flow microcalorimetry, Immobilized biocatalysts, Reaction rate monitoring, Investigation of kinetic properties, Characterization of bioaffinity systems. 

CSPs Based on Target-specific Bioaffinity Systems... 

These mutually interacting molecules occur naturally in organisms (except analogues) and thus may be called bioaffinity systems. In contrast, similar artificial systems exist which mimic these natural systems and they may also be utilized. Such frequently used couples are ... 

Reviews of w/o-ME-based LLE of biomolecules are readily available [4,57,102-104]. However, new results have been generated in this field since the publication of the cited reviews. For instance, there has been a large amount of research involving new surfactant and surfactant systems, particularly those involving nonionic and natural surfactants such as phosphatidylcholine and bioaffinity surfactants (Table 1), in order to increase biocompatibility and selectivity and prevent denaturation that occurs using ionic surfactants. The more recent results along these lines will be presented here, along with an overview of the LLM process. 

Immobilization provides great flexibility, particularly in the design of enzyme-based bioanalytical systems. Some relatively recent developments in protein immobilization methodology which can be broadly classified as reversible immobilization methods, have resulted in novel analytical approaches such as bioaffinity sensors (7) and flow injection binding reactions (8). 

In this section we discuss the choice of a chromatographic system on the basis of, firstly, the type of stationary phase and, secondly, the composition of the eluent. A division in subsections is made from the kinds of stationary phases available, bearing in mind that the more specialized phases for ion-exchange, gel permeation (size exclusion), and bioaffinity chromatography are treated in separate chapters (4.5 to... 

Bioaffinity Sensing Based on the Avidin-Biotin System. Avidin is another biotin-binding protein that has been widely used in assays. Its affinity for biotin (Aatf = 1.7 X 10 M" ) is even higher than that of streptavidin (Katr = 2.5 x 10 M" ). To determine whether streptavidin or avidin is the better protein to use in PLL-g-PEG/PEGbiotin-based immunoassays, the experiments described in section 3.2 were repeated with avidin taking the place of streptavidin. 

---