Bioaffinity elution

Bioaffinity elution (also known as substrate or biospecific elution) was described by Pogell [155], and later by Haar [156], and has recently been reviewed by Scopes... 

A widely applicable, non-chaotropic elution technique in bioaffinity chromatography is electrophoretic desorption [60,74,75]. If the affinant is coupled to the solid support by an azo bond or by thiol- or alcohol-ester bonds, the complex of the affinant with the isolated substance can be detached from the solid matrix and then the affinity ligand separated by dialysis or gel filtration. 

Abstract Target-specific chiral selectors, which are characterized by a predictable elution order depending on the target enantiomer empioyed for the selection of the chirai selector, have recently received much attention in the enantioselective analysis fieid. In this context, bioaffinity-based molecular recognition toois such as nucleic acid aptamers have notabiy demonstrated very attractive features for the chiral discrimination of active moiecuies. In this chapter, the enantioseiective properties of aptamer chiral selectors and the major factors that control and modulate the liquid chromatography and capillary electrophoresis enantiomer separation are addressed. 

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