Basic pancreatic trypsin inhibitor BPTI

After a demonstration of the method s abilities on simulated data,8 the algorithm was soon applied to several cases with real data such as metal-lothionein,32 tendamistat,33 and basic pancreatic trypsin inhibitor (BPTI).34 Furthermore, aside from the original program, DISMAN,8 the basic algorithm has been implemented in other programs such as DADAS35 and the... 

For example, from protein basic pancreatic trypsin inhibitor (BPTI), four experimental ISNets are observed from a double quantum filtered COSY spectrum as shown at the bottom of Fig. 6. No one of them is completely matched onto its ISNet cluster center. 

BPTI, basic pancreatic trypsin inhibitor HPI, isoinhibitor K from Helix pomatia (Tschesche and Dietl,... 

In this paper an outline of the method will be given. Both technical and chemical aspects of the generation and detection of the polarization effects will be discussed. The CIDNP behaviour of the aromatic amino acids provides the basic Information necessary for applying the method to protein systems. The proteins that will be considered are the bovine pancreatic trypsin Inhibitor (BPTI), HEW lysozyme, and rlbonuclease A and S. These proteins have been extensively studied by nmr and X-ray crystallographic methods and may therefore suitably serve as model systems. Since the active sites of enzymes are necessarily accessible (at least to substrates ) there Is a good chance of observing CIDNP In active site residues. Examples of this will be given In the cases of both rlbonuclease and lysozyme. 

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