Basic Considerations of Liquid Chromatography

Identifying the most suitable separation conditions is the main objective of separation scientists. It is easier for skilled chromatographers, but this is a complicated subject for beginners. One approach is to find a chromatogram that exhibits the separation of a similar mixture. However, similar mixtures may have been separated under very different conditions either the separation 

Basic Concepts of High-performance Liquid Chromatography 

Choosing the sample pre-treatment method is difficult. The most important consideration is the final condition of the target compounds. What kind of solution is obtained The type of solvent and the concentration of the target compounds are very important in the selection of the separation conditions. 

The separation conditions employed for size-exclusion liquid chromatography are simple. A strong solvent for analytes and a suitable stationary phase 

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