Chondroitin sulfate proteoglycans basement membranes

Chondroitin sulfate was identified in embryonic basement membrane on the basis of studies showing the susceptibility of sulfate-labeled material to chondroitinase and a reaction with monoclonal antibodies (Couchman et al., 1984 Paulsson et al., 1985b). Similarly, Reichert s membrane, endothelial L2, PYS cells, EHS tumor cells, and isolated glomeruli produce variable amounts of these proteoglycan (Oohira et al., 1983 Stow et al., 1983 Oldberg et al., 1981 Tyree et al., 1984 Hassell et al., 1980). The molecule produced by L2 cells (Mr 40K) contains a small protein core with a unique amino acid sequence (Bourdon et al., 1985). It appears that in such systems, little of the chondroitin sulfate proteoglycan is deposited in the matrix. 

Sannes PL, Burch KK, Khosla J, McCarthy KJ, Couchman JR. Immunohis-tochemical localization of chondroitin sulfate, chondroitin sulfate proteoglycan, heparan sulfate proteoglycan, entactin, and laminin in basement membranes of postnatal developing and adult rat lungs. Am J Respir Cell Mol Biol 1993 8 245-251. 

Carlson SS, Iwata M, Wight TN. A chondroitin sulfate/keratan sulfate proteoglycan, PG-1000, forms complexes which are concentrated in the reticular laminae of electric organ basement membranes. Matrix Biol 1996 15 281-292. 

---