Base-pair mutations

RUM H., Nielsen P.E., Egholm M., Berg R.H., Buchardt O., Stanley C. Single base pair mutation analysis by PNA directed PCR clamping. Nucleic Acids Res. 1993 21 5332-5336. 

Thier, R., Taylor, J.B., Pemble, S.E.. Humphreys, G, Persmark. M., Ketterer, B. Guengerich. F.P. (1993) Expression of mammalian glutathione S-transferase 5-5 in Salmonella typhimurium TA1535 leads to base-pair mutations upon exposure to dihalomethanes. Proc. nat Acad. Sci. USA, 90, 8576-8580... 

Li, Wu, and Luo s weighted pathway Changes weighted as nondegenerate, 2-fold degenerate, or 4-fold degenerate sites, and by expected to observed frequenries of base pair mutations 13... 

Polymerase chain reaction is particularly useful for genetic analysis because both amplification and primer specific isolation of gene fragments occur simultaneously. Allele-specific amplification can be employed to detect a single base-pair mutation through the use of a specially designed primer which is complementary to... 

The test strains include those that are able to detect both frameshift and base-pair mutations. 

The platinum-DNA adduct that is the major source of mutation induction by cis-DDP is a matter of controversy. One study (17) found platinated d(GpNpG) sequences to be the most mutagenic loci for base pair substitution. Another (18) reported that d(ApG) and d(GpG) cross-links accounted for 90% of single base pair mutations, and that d(ApG) crosslinks were five times more mutagenic. This conflict may be due to the different systems used for these studies, and the range of mutations they detect. Site-specifically modified M13 genomes were constructed in part to answer the question of the mutability of individual platinum-DNA adducts... 

About half of those spontaneous abortions have a recognizable (c)d o)genetic problem in the form of chromosomal anomalies that are big enough to see in a microscope with proper preparation and staining. The other half of them have had no readily diagnosable problem. Recent advances in DNA microarray technology now allow us to see sub-microscopic anomalies in the DNA of some of them, and even to find some of the single-base-pair mutations when we have a reasonable idea of what to look for. 

Nelson, P. S., Frye, R. A., and Liu, E. (1989) Bifunctional oligonucleotide probes synthesized using a novel CPG support are able to detect single base pair mutations. Nucl. Acids Res 17, 7187-7194. 

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