Bacteriophage cloning vectors

PAC A high capacity (70-95 kb) cloning vector based upon the lytic E. colt bacteriophage PI that replicates in bacteria as an extrachromosomal element. 

An essential feature of the cloning vector used is that it must be capable of self-replication in the cell into which it is introduced, which is usually E. coli. Two of the most commonly used types of vector in conjunction with E. coli are plasmids and bacteriophage X. Plasmids are circular extra-chromosomal DNA molecules, generally between 5000 and 350 0000 bp in length, that are found naturally in a wide range of bacteria. They generally house several... 

The principles that govern the delivery of recombinant DNA in clonable form to a host cell, and its subsequent amplification in the host, are well illustrated by considering three popular cloning vectors commonly used in experiments with E. coli—plasmids, bacteriophages, and bacterial artificial chromosomes—and a vector used to clone large DNA segments in yeast. 

Cloning vectors include plasmids, bacteriophages, and, for the longest DNA inserts, bacterial artificial chromosomes (BACs) and yeast artificial chromosomes (YACs). 

Bacteriophage A Vectors Are Useful for Cloning Larger DNA Segments... 

Collins, J. Hohn, B. (1978). Cosmids a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage X heads. Proc. Natl. Acad. Sci. USA 75,4242-4246. 

When a bacteriophage is used as the cloning vector, the gene library is screened as an array of plaques in a bacterial lawn. A hybridization screening method is used similar to that described for plasmid screening above in this case the replica filter is called a plaque lift. ... 

Various bacterial plasmids, bacteriophages, and yeast artificial chromosomes are used as cloning vectors (Brown, 2001). At the heart of the general approach to generating and propagating a recombinant DNA molecule is a set of enzymes which synthesize, modify, cut, and join DNA molecules. 

Collins, J., and Hohn, B. (1978). Cosmids A Type of Plasmid Gene-Cloning Vector That Is Packageable in Vitro in Bacteriophage A Heads. Proc Natl Acad Sci 75 4242. 

Figures- a Types of cloning vectors a bacteriophage and a plasmid.

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