Bacteriophage T4 DNA

The renaturation rate of DNA is an excellent indicator of the sequence complexity of DNA. For example, bacteriophage T4 DNA contains about 2 X 10 nucleotide pairs, whereas Escherichia coli DNA possesses 4.64 X 10 . E. coli DNA is considerably more complex in that it encodes more information. Expressed another way, for any given amount of DNA (in grams), the sequences represented in an E. coli sample are more heterogeneous, that is, more dissimilar from one another, than those in an equal weight of phage T4 DNA. Therefore, it will take the E. coli DNA strands longer to find their complementary partners and reanneal. This situation can be analyzed quantitatively. 

Alley, S.C., Ishmael, F.T., Jones, A.D., and Benkovic, S.J. (2000) Mapping protein-protein interactions in the bacteriophage T4 DNA polymerase holoenzyme using a novel trifunctional photo-cross-linking and affinity reagent./. Am. Chem. Soc. 122, 6126-6127. 

Tuerk, C. Gold, L. Systematic evolution of ligands by exponential enrichment RNA ligands to bacteriophage T4 DNA polymerase. Science 1990, 249, 505-510. 

Frey, M. W., Sowers, L. C., Millar, D. P., and Benkovic, S. J. (1995). The nucleotide analog 2-aminopurine as a spectroscopic probe of nucleotide incorporation by the Klenow fragment of Escherichia coli polymerase I and bacteriophage T4 DNA polymerase. Biochemistry 34, 9185-9192. 

Gillin, F. D., and Nossal, N. G. (1976). Control of mutation frequency by bacteriophage T4 DNA polymerase II. Accuracy of nucleotide selection by the L88 mutator, CB120 antimutator, and wild type phage T4 DNA polymerases. J. Biol. Chem. 251, 5225-5232. 

Mandal, S. S., Fidalgo da Silva, E., and Reha-Krantz, L. J. (2002). Using 2-aminopurine fluorescence to detect base unstacking in the template strand during nucleotide incorporation by the bacteriophage T4 DNA polymerase. Biochemistry 41, 4399—4406. 

Tuerk C, Eddy S, Parma D, Gold L, The autogenous translational operator recognized by bacteriophage T4 DNA polymerase, J. Mol. Biol., 213 749-761, 1990. 

Reassociation Cot curves for bacteriophage T4 DNA, E. coli DNA, and mouse DNA. [Reproduced with permission from D. M. Prescott Cells Principles of Molecular Structure and Function. Jones and Bartlett, London, 1988.]... 

Tuerk C, Gold L. Systematic Evolution of Ligands by Exponential Enrichment RNA Ligands to Bacteriophage T4 DNA Polymerase. Science 1990 249 505-510. 

See Tuerk, C., Gold, L. Systematic evolution of ligands by exponential enrichment RNA ligands to bacteriophage T4 DNA polymerase. Science 1990, 249, 505-510 and Ellington, A.D., Szostak, J.W. In vitro selection of RNA molecules that bind specific ligands. Nature 1990, 346, 818-822. 

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