Bacteriophage screening

Using various random-peptide screening strategies, several groups have recently identified linear polypeptide seqnences that bind to heterotrimeric G-protein subunits in unique and informative ways (5, 13, 29, 30). One example is KB-1753, a 19-amino acid peptide derived from bacteriophage screening... 

The next step is to screen the library for the insert of interest (Fig. 6.4). The bacteriophages are mixed with bacteria at the appropriate concentration such that each bacteriophage will infect a single bacteria. The progeny will infect adjacent bacteria and repeat the process of infection, replication and bursting until... 

M13mpl8 Filamentous E. coli DNA sequencing, mutagenesis, lac screening bacteriophage... 

XZAP Bacteriophage E. coli Resistance to ampicillin. lac screening. cDNA cloning, gene expression, automatic excision into plasmid pBluescript SK... 

Heinemann, B. Howard, A.J. (1964) Induction of lambda-bacteriophage in Escherichia coli as a screening test for potential antitumor agents. Appl. Microbiol., 12, 234-239... 

When a bacteriophage is used as the cloning vector, the gene library is screened as an array of plaques in a bacterial lawn. A hybridization screening method is used similar to that described for plasmid screening above in this case the replica filter is called a plaque lift. ... 

Christian RB, Zuckermann RN, Kerr JM, Wang L, Malcom BA, Simplified methods for construction, assessment and rapid screening of peptide libraries in bacteriophage, J. Mol. Biol., 227 711-718, 1992. 

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