Bacteriophage insert size

Pl-derived artificial chromosome. (PAC). One type of vector used to clone DNA fragments (100- to 300-kb insert size average, 150 kb) in Escherichia coli cells. Based on bacteriophage (a virus) PI genome. 

Yeast (Saeeharomyees eerevisiae) has a genome size of 1.21 X 10 bp. If a genomic library of yeast DNA was constructed in a bacteriophage A vector capable of carrying 16-kbp inserts, how many indi-... 

The structures of ssDNA bacteriophages ( X174 and G4 have been reported in mature and provirus forms (Dokland et al, 1997 McKenna et al, 1992b, 1996). The mature viruses are T=1 with 60 copies each of the F, G, and (small J) proteins, and 12 copies of the H protein. Both the F and G proteins are classic viral jelly-roll structures. It is the F protein that occupies the positions homologous to the (+)ssRNA capsids. At 430 residues, the F protein is closest in size to the parvoviral capsids, and achieves its size through large loop insertions, primarily in the EF and HI... 

RNase protection assays (RPA) are based on the property of RNase to digest ii RNA, but not ds RNA, and its principles and applications resemble those of SI analysis (Lynn et al., 1983 Zinn et at, 1983) (Fig. 12.3). The sequence of interest is inserted in a plasmid downstream of a bacteriophage promoter (e.g., pUC118, pT7, etc.. Table 4.4). The purified plasmid is then restricted downstream of the inserted DNA and the linearized plasmid is transcribed in the presence of a labeled rNTP precursor. The transcript should be complementary to the RNA to be studied and an excess of probe is hybridized to its target. Any RNA remaining ss is then digested by one or more RNases. The size of the RNase-resistant probe and the... 

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