Bacteriophage infection/infectivity

Gomila, M., Solis, J. J., David, Z., Ramon, C., and Lalucat, J. (2008). Comparative reductions of bacterial indicators, bacteriophage-infecting enteric bacteria and enteroviruses in waste-water tertiary treatments by lagooning and UV-radiation. Water Sci. Technol. 58, 2223-2233. 

The first step in bacteriophage infection is the adsorption of the phage to a receptor on the bacterium. Almost every structure on the surface of a bacterial cell, or extending from it, can act as (or include) phage receptors (29). The 0-antigenic polysaccharide chains of the LPS are no exgeption in this respect. Since it has been estimated that 10 > to 10 LPS molecules are found on each bacterial cell (30), it is evident that part of the... 

FIGURE 2.7 Life-reproductive cycle of a virus as illustrated by a T-even bacteriophage infecting a bacterial cell (a) dormancy, (b) adsorption, (c) penetration, (d) replication of new proteins and nucleic acids, (e) maturation, and (f) release and bursting of hold cell. 

Fig. 10.23. Gel electrophoretic analysis of RNA synthesis in T5-bacteriophage infected . coli cells. After pulse-labeling with for one minute the RNA was analysed electrophoretically at the times indicated. The profiles are densitometry traces of autoradiographs of the gels. These are used for the metabolic identification of the different classes of RNA synthesised (Sirbasku and Buchanan, 1970).

To complete the library construction, the heteroduplex CCC-dsDNA must be introduced into an E. eoli host that contains an F episome to enable M13 bacteriophage infection and propagation. Phage-displayed library diversities are limited by methods for introducing DNA into E. eoli, with the most efficient method being high-voltage electroporation. 

Bacteria use restriction enzymes to destroy invading, exogenous DNA for instance, DNA injected during bacteriophage infection. How can the restriction enzyme hydrolyze the foreign DNA and not destroy the DNA of the bacterium in which it resides ... 

Rapid detection of bacteriophage infection and prophage induction using electric biochips. Microb. Cell Fact, 5,... 

Keis, S. (1992) Characterization of two bacteriophages infecting Clostridium acetobutylicum. M.Sc. thesis. University of Otago. 

Gold, W. and Watson, D.W. (1950) Studies on the bacteriophage infection cycle I. a lytic activity assay for bacteriophages of Clostridium madisonii. J. Bacterial., 59, 13-16. 

Lytic cycle The sequence of events in which a bacteriophage infects a bacterial cell, replicates, and eventually causes lysis of the cell. 

Infection. For the process of bacteriophage infection, see Phage development. 

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