Bacterial strains characterization

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As with inhibitors produced by eukaryotes, few studies have characterized and quantified inhibitors produced by natural biofilms. Rather, most studies have focused on isolated bacterial strains, or biofilms, cultured in laboratories.98,100-103 Only one inhibitory metabolite from biofilms has been fully characterized, the natural product ubiquinone-8 from the culture supernatant of the bacteria Alteromonas sp.,103 a bacterium isolated from the sponge Halichondria okadai. 

The fundamental need to characterise and quantify bacterial strains in natural biofilms in order to understand their potential role as producers of settlement signals highlights the need for molecular techniques which can be used to identify and quantify bacteria in situ. For example, low abundance of putatively deterrent bacterium on surfaces in situ may preclude the production of sufficient quantities of deterrent metabolites. Appropriate techniques for such characterization include denaturing gradient gel electrophoresis (DGGE)107 and fluorescence in situ hybridization (FISH)108 and would in principle allow for the detection and quantification of all species in the biofilm. 

Characterization and Identification of Bacterial Strains Isolated From BC1. 

Natural product extracts and bacterial culture collections do not necessarily work well together on drug discovery platforms. The separation, identification, characterization, scale-up, and purification of natural products for large-scale libraries suitable for these HTS are daunting, and rational arguments for the selection of organisms and/or natural product molecules are often absent, especially given the poor taxonomic characterization of strains in natural product bacterial strain collections. 

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