Bacteria PHAs produced

Bacteria PHA produced Substrates Special feature Reference... 

In this article, we review the development of processes for the production of RHAs in natural PHA-producing bacteria and metabolically engineered E. coli strains. 

The key concept consists of PHA biosynthesis and subsequent depolymerization of produced PHA in cells therefore, it canbe called an in vivo PHA depolymerization process (15). RHAs produced by the depolymerization of PHAs can be excreted into the medium when the further intracellular metabolism of RHAs is inhibited. Because all the PHA-producing bacteria also possess PHA degradation pathways, in vivo depolymerization of PHA can be achieved by natural PHA-producing bacteria when the proper conditions are met. 

We have recently reported the development of a process for the in vivo depolymerization of PHA in several natural PHA producing bacteria including A. latus, R. eutropha, and pseudomonads (15). Metabolic pathways involved in the synthesis and degradation of PHB are shown in Fig. 1. 

Electron microscopy has been employed to identify the locations, sizes, and number of granules in cyanobacteria. This technique revealed interesting observation whereby the number and sizes of PHA granules were comparable to that of some PHA-producing bacteria (Sudesh et al. 2001). In addition, freeze-fracture electron microscopy observation on Synechocystis sp PCC 6803 showed that the internal microstructure of P(3HB) was essentially similar to the P(3HB) granules... 

Ali N, Suhaimi NS (2009) Performance evaluation of locally fabricated asymmetric nanofiltration membrane for Batik industry effluent. World Appl Sci J 5 46-52 Alias Z, Tan IKP (2005) Isolation of palm oil-utilising, polyhydroxyalkanoate (PHA)-producing bacteria by an enrichment technique. Bioresour Technol 96 1229-1234 Allen AD, Anderson WA, Ayorinde FO, Eribo BE (2010) Biosynthesis and characterization of copolymer poly(3HB-co-3HV) from saponified Jatropha curcas oil by Pseudomonas oleovorans. J Ind Microbiol Biotechnol 37 849-856 Allen AD, Anderson WA, Ayorinde F, Eribo BE (2011) Isolation and characterization of an extracellular thermoalkanophilic P(3HB-co-3HV) depolymerase from Streptomyces sp. INI. Int Biodeterior Biodegrad 65 777-785... 

Researchers in industry have also reported working on methods with which transgenic crops will be developed that express PHA synthesis routes from bacteria to produce PHA as energy storage in their tissues [16]. 

Staphylococcus, and so on [1-3, 6, 7]. This chapter will highlight the development of the isolation and screening methods employed in the discovery of PHA producers, as well as the occurrence of newly isolated PHA-producing bacteria from various ecosystems and their ability to utilise renewable feedstocks. 

Gasser and co-workers [12] applied cultivation-dependent (lipophilic staining and PCR) and independent [single-strand conformation polymorphism (SSCP)] methods to analyse the occurrence and diversity of PHA-producing bacteria from the plant-associated... 

Shrivastav and co-workers [26] attempted to isolate PHA-producing bacteria from soil and marine environments using a Jatropha biodiesel by-product generated from the biodiesel production process which uses Jatropha curcas seeds as the carbon source. The Nile red... 

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