B. subtilis phages

In principle, the in vitro synthesis of phage enzymes in an E, coli system can be applied to both, E. coli and non-E. coli phage. Enzyme synthesis was observed using DNAs from various B. subtilis phage (j)29 lysozyme (in col-... 

Table 1. Some Biological Properties of Transfecting B, subtilis Phage and their DNA (References in Parentheses)...

Fig, 3. Competent cells of B. subtilis were mixed with DNA from various B. subtilis phages or transforming DNA at time zero and incubated at 37°. At various subsequent times, DNAse was added, the mixture was incubated for another 5 min and plated with indicator bacteria to determine transfection cells were plated on minimal and complete media to determine transformation. The transfection or transformation value obtained at 40 min is set at 100%. Fig. 3a shows the following uptake curves 0---0 transformation try- ->try+ by try+ DNA --------- 029 DNA, --------- SPP 1 DNA, A----------A... 

Rottlander, E., Trautner, T. A. Genetic and transfection studies with B. subtilis phage SP50. I. Phage mutants with restricted growth on B. subtilis strain I68. Molec. gen. Genet. 108, 47-60 (1970). 

Trilling and Aposhian have partially purified a DNase from extracts of B. subtilis infected with phage SP-3 (35). This enzyme requires magnesium ion and show s optimal activity between pH 7.8 and 8.9 in Tris buffers. It is highly specific for denatured DNA and appears to catalyze a unique type of exonucleolytic attack beginning at the 5 end of the chain which sequentially releases dinucleotides. Neither mono-... 

Different bacteriophages have been used to transduce noncompetent B. subtilis strains. Detailed protocols for transduction with the PBSl and SPPl phages... 

We have extensive test data where we challenged nano alirmina filters with various bacteria (E. coli, R. terrigina, B. globiggi, B. diminuta). We found that all such bacteria in the presence of a food source, can proliferate on the filter, and we therefore presume the filters are compatible with bacteria. We have also collected MS2 vims on NanoCeram and have been able to recover -90% of viable phage. The toxicity of raw nano alumina fiber towards E. coli, Staphylococcus aureus, B. subtilis, B. pumilis, and Candida albicans has been... 

The dcm ORF contains 1416 nt. [Note The dcm genes of B. subtilis temperate phages have also been cloned and sequenced (36).]... 

B, subtilis strain l68, originally described by Burkholder and Giles (1947), and its derivatives and B, amyloliquefaciens (Welker and Campbell, 1967) (formerly strain H. Reilly and Spizizen, 1965) are the bacteria which have been used as hosts for transfection. Individual laboratories have isolated their own strains as appropriate for particular purposes. For some transfection experiments it is convenient to use host cells which are resistant to the adsorption of the phage particles whose DNA is being investigated. 

Marker rescue systems have proved particularly useful in the investigation of a number of biological problems. These techniques offer a sensitive and specific tool for detecting and quantitating small amounts of phage DNA in the presence of an excess of other DNAs. Thus Klotz and Spatz (1971) have shown that SPP 1 replication in infected B. subtilis cells proceeds in an exponential fashion. 

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